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Benedict's Test for Reducing Sugars

Tests for glucose, sucrose, and fructose.

Benedict's Test for Glucose


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2 MB
31 seconds

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5 Slides



     
Glucose is heated with Benedict's reagent to form a brick red precipitate.

Benedict's Test for Sucrose


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.9 MB
13 seconds

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Sucrose is heated with Benedict's reagent with no visible reaction.

Benedict's Test for Fructose


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1 MB
14 seconds

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Fructose is heated with Benedict's reagent to form a red precipitate.

Clinitest Tablet is Added to Glucose


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1.1 MB
18 seconds

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A Clinitest tablet (anhydrous Benedict's reagent) is added to a solution of glucose producing a precipitate of brick-red Cu2O.

Clinistrip Test of Glucose is Demonstrated


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.5 MB
7 seconds

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A Clinistrip for measures levels of glucose in urine.

Discussion
Glucose is heated with Benedict's reagent (CuSO4, NaOH, and tartaric acid) to form a brick red precipitate. Sucrose does not react under these conditions. Fructose does form a red precipitate with Benedict's reagent.

Carbohydrates that contain aldehydes or a-hydroxymethyl ketones can be oxidized by Cu(II) ion and are classified as reducing sugars. They reduce the Cu(II) ion to Cu(I).



       

Citation:  
  Shriner, R. L.; Fuson, R. C.; Curtin, D. Y.; Morrill, T. C. The Systematic Identification of Organic Compounds; 6th ed.; Wiley: New York, 1980; pp 172.