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| Benedict's Test for Reducing Sugars |
Discussion
Glucose is heated with Benedict's reagent (CuSO4, NaOH, and tartaric acid) to form a brick red precipitate. Sucrose does not react under these conditions. Fructose does form a red precipitate with Benedict's reagent.
Carbohydrates that contain aldehydes or a-hydroxymethyl ketones can be oxidized by Cu(II) ion and are classified as reducing sugars. They reduce the Cu(II) ion to Cu(I).
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Citation:
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Shriner, R. L.; Fuson, R. C.; Curtin, D. Y.; Morrill, T. C.
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The Systematic Identification of Organic Compounds; 6th ed.; Wiley: New York, 1980; pp 172.
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