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Benedict's Test for Glucose

Glucose is heated with Benedict's reagent to form a brick red precipitate.

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2 MB, 31 seconds

File: MOVIES/TRAM18/0591308.MOV

Voiceover
Benedict's reagent is a solution of copper sulfate, sodium hydroxide, and tartaric acid. Aqueous glucose is mixed with Benedict's reagent and heated. The reaction reduces the blue copper (II) ion to form a brick red precipitate of copper (I) oxide. Because of this, glucose is classified as a reducing sugar.

Discussion
Aqueous glucose is mixed with Benedict's reagent, a solution of copper sulfate, sodium hydroxide, and tartaric acid. The mixture is heated. Carbohydrates which react with Benedict's reagent to reduce the blue copper (II) ion to form a brick red precipitate of copper (I) oxide are classified as reducing sugars.


       

Results of Benedict's Test with Glucose, Sucrose, and Fructose
Design, Text and Demonstrator:  
  Gary Trammell University of Illinois at Springfield, Springfield, IL 62794
Videographer/Editor:  
  Steve Dykema University of Illinois at Springfield, Springfield, IL 62794
Voice:  
  Margaret Biddle University of Wisconsin - Madison, Madison, WI 53706
Audio Production:  
  Greg Minix University of Wisconsin - Madison, College of Engineering, Madison, WI 53706
  Jerrold J. Jacobsen University of Wisconsin - Madison, Madison, WI 53706