Fill the Cuvet with the Sample
After the spectrophotometer has been zeroed, remove the reference sample, pour out the reference solution, rinse the cuvet three times with the sample.
Place the Cuvet in the Cell Compartment
Place the cuvet in the cell compartment. Again, be sure that the clear faces of the cuvet are perpendicular to the light source and that the mark is aligned in the same position.
Collect Absorbance versus Wavelength Data
Close the lid, and use the computer or keyboard controls on the spectrophotometer to begin scanning the wavelengths and recording absorbance measurements. Once the instrument is finished collecting data, prepare a plot of absorbance versus wavelength.
If the spectrum goes off the scale, dilute the sample solution and re-collect the spectrum.
