Make sure there is enough liquid in a beaker or other container to completely fill the pipet. (NEVER pipet directly from a reagent bottle or volumetric flask.)
Carefully place the attachment of the three-way bulb over the mouth of the pipet.
Squeeze the air valve (A) and the bulb simultaneously to empty the bulb of air. Then, release the air valve (A).
Place the tip of the pipet below the liquid's surface.
Gently squeeze the suction valve (S) to draw liquid into the pipet. When the liquid is above the desired volume, stop squeezing the suction valve. DO NOT remove the three-way bulb from the pipet. DO NOT ALLOW THE LIQUID TO ENTER THE BULB.
If the level of the liquid is not high enough squeeze the air valve (A) and the bulb again to expel air from the bulb. Draw more liquid by squeezing the suction valve (S).
Touch the tip of the pipet to the inside of the beaker to remove the drop hanging from the tip. Observe and record the initial volume.
To transfer the liquid to a receiving vessel, press the empty valve (E) until the desired volume is delivered. Remember to touch the tip of the pipet to the inside wall of the receiving vessel.
