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| Hydrolyzing Sucrose with Invertase |
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Narrative Sucrose is added to two beakers containing an acetate buffer. Invertase is added to one of the beakers. Invertase hydrolyzes sucrose into its monosaccharides, glucose and fructose. Later, hot Fehling's reagent is used to test for the presence of glucose. The sucrose solution that did not have invertase does not react with Fehling's reagent. The sucrose solution with invertase reacts to form a brick red precipitate of copper(I) oxide. This confirms that sucrose was hydrolyzed by invertase.
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