Hydrolyzing Sucrose with Invertase

CCA! Volume 5

Home

Table of Contents

Keyword Index

Textbook X-ref

About CCA!

Hydrolyzing Sucrose with Invertase

Invertase hydrolyses sucrose into glucose and fructose. The hydrolysis is confirmed using Fehling's reagent.


View Slide Thumbnails		View Next Movie

3.2 MB, 57 seconds

File: MOVIES/KJBIOCHEM/HYDROSUC.MOV

Voiceover
Sucrose is added to two beakers containing an acetate buffer. Invertase is added to one of the beakers. Invertase hydrolyzes sucrose into its monosaccarides, glucose and fructose. Later, hot Fehling's reagent is used to test for the presence of glucose. The sucrose solution that did not have invertase does not react with Fehling's reagent. The sucrose solution with invertase reacts to form a brick red precipitate of copper(I) oxide. This confirms that sucrose was hydrolyzed by invertase.

Design, Text, and Demonstration:
	Kristin Johnson	University of Wisconsin - Madison, Madison, WI 53706

Video production, editing, and voice:
	Jerrold J. Jacobsen	University of Wisconsin - Madison, Madison, WI 53706
	Greg Minix	University of Wisconsin - Madison, College of Engineering, Madison, WI 53706
	Michael Killips	University of Wisconsin - Madison, School of Nursing, Madison, WI 53706