Fehling's Test for Reducing Sugars

CCA! Volume 5

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Fehling's Test for Reducing Sugars

Glucose reduces blue Fehling's reagent to form copper(I) oxide. Sucrose does not reduce Fehling's reagent.


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Voiceover
Blue copper sulfate is mixed with a solution of potassium hydroxide and potassium sodium tartrate to form a deep blue solution of Fehling's reagent. The solution is heated on a hot plate. Later, sucrose is added and no change is observed. On the other hand, glucose, a reducing sugar, reacts with Fehling's reagent to form a brick red precipitate of copper(I) oxide.

Design, Text, and Demonstration:
	Kristin Johnson	University of Wisconsin - Madison, Madison, WI 53706

Video production, editing, and voice:
	Jerrold J. Jacobsen	University of Wisconsin - Madison, Madison, WI 53706
	Greg Minix	University of Wisconsin - Madison, College of Engineering, Madison, WI 53706
	Michael Killips	University of Wisconsin - Madison, School of Nursing, Madison, WI 53706