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Movie Size - 1 MB Movie Duration - 36 Seconds File - CCA6/MOVIES/Labcurrent/pipetvol/FILLPIP.MOV

Discussion

1. Make sure there is enough liquid in a beaker or other container to completely fill the pipet. (NEVER pipet directly from a reagent bottle or volumetric flask.)
2. Place the tip of the pipet below the liquid's surface. Using a bulb or pump, draw the liquid into the pipet. Draw slightly more liquid than needed, but DO NOT allow the liquid to enter the bulb. Lift the pipet from the solution and touch the tip of the pipet to the side of the container to remove any excess liquid that is outside the pipet.
3. Any excess liquid that you fail to touch off when filling the pipet might be transferred to the receiving vessel and needs to be removed before delivering the liquid. Failure to touch off the excess liquid will result in poor results because the volume transferred will be more than the recorded volume.
4. Drain the liquid into a waste beaker until the bottom of the meniscus is aligned with the graduation mark. Remember to eliminate parallax when aligning the meniscus with the graduation mark.

Narrative

Use a pipet bulb to draw liquid into the pipet past the mark on the neck. Wipe off the outside of the tip with a clean towel. Drain the liquid into a waste container. Use your finger or thumb to control the flow of liquid from the pipet. Align the bottom of the meniscus with the mark on the neck. Touch off the hanging drop.