Thin Layer Chromatography of Dyes

CCA! Volume 5

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Thin Layer Chromatography of Dyes

Sudan B, Erythrosin, Rhodamine B, and a mixture of the three are spotted on a silica gel thin layer chromatography plate, and developed in acetone to separate the components.


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Voiceover
A silica gel thin layer chromatography plate is spotted with the dyes Sudan B, Erythrosin, Rhodamine B, and a mixture of all three. The plate is placed in a tank containing acetone and developed to separate the components.

Discussion

The solvent front is marked and a ruler is held next to the plate to allow calculation of Rf values.

The plate is examined first under short wave ...

... then long wave ultraviolet light.

Rhodamine B fluoresces strongly under long wave U.V. light.

	distance component travels
Rf =	-------------------------------
	distant solvent travels

Citation:
		Eastman Fine Chemicals News, Eastman Kodak Co., 1991, 62, pp 9-10.

Design, Text and Demonstrator:
	Gary Trammell	University of Illinois at Springfield, Springfield, IL 62794

Videographer/Editor:
	Steve Dykema	University of Illinois at Springfield, Springfield, IL 62794

Voice:
	Margaret Biddle	University of Wisconsin - Madison, Madison, WI 53706

Audio Production:
	Greg Minix	University of Wisconsin - Madison, College of Engineering, Madison, WI 53706
	Jerrold J. Jacobsen	University of Wisconsin - Madison, Madison, WI 53706